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Fractions containing SF3B1 were pooled together, washed with PBS and concentrated in an Amicon Ultra-4 Centrifugal Filter Unit (Sigma-Aldrich, UFC801024)

Fibrinogen and Fibrin Induce Synthesis of Proinflammatory Cytokines From Isolated Peripheral Blood Mononuclear Cells

doi:10.1586/14787210.2015.1064309 51

However, avoid contact with the eyes