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IVW combines SNP-specific estimates calculated using Wald ratios, assuming no directional pleiotropy of each SNP ( 2.4.2 Mediation analysis A two-step MR analysis (product of coefficients method) was applied to assess mediation ( Supplementary Figure 1

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Raufman and Eng's research followed in the footpath of Italian pharmacologists who had previously isolated a number of exciting biologically active peptides able to activate human receptors in lower animals like the frog usually found in their skin secretions (Holst 2024)

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