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b , Immunoblot of SLC25A39, SLC25A12 and CS in HeLa cells treated with the indicated doses of BSO for 24 h

Culture conditions Yeast cells were aerobically cultivated in YPE medium (1% (w/v) yeast extract, 2% (w/v) bactopeptone and 3% (v/v) ethanol) in the absence and presence of 15 mM aspirin (acetylsalicylic acid, ASA) (Sigma-Aldrich), at 28 C with constant shaking at 250 rpm