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In another in vivo study, a reduction in levels of testosterone and an increase in levels of LH and FSH were seen after NAC supplementation among adult male Wistar rats (Reference Moghadam, Shariati and Naeimi16)
Cell 133 , 235249 (2008)
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The recycling reaction was initiated by the addition of reaction buffer (100 mM sodium phosphate, pH 7.4), supplemented with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) (100 M) (Sigma), NADPH (100 M) (Roth), glutathione reductase (1 U/ml) (Sigma), and EDTA (1 mM) (Sigma)
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