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Proliferation Assay Fresh isolated PBMCs or purified T/B cells labeled carboxyfluorescein succinimidyl ester (CFSE) were cultured with different concentration of L-Cn (Sigma-Aldrich) for 5 days in the presence of soluble anti-CD3 (5 g/mL, Biolegend) and anti-CD28 (5 g/mL, Biolegend) for T cell proliferation assay or 7 days in the presence of CPG (10 g/mL, InvivoGen) for B cell proliferation assay or with medium only as control

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Changes in appetite and body weight in response to long-term oral administration of the ghrelin agonist GHRP-2 in growth hormone deficient children