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The probes were annealed to the complementary toehold sequences that also carry a 10 to 12 bp overhang of A-repeats (Supplemental Table 1)

Mechanistically, this complementarity is plausible: while surgery provides mechanical gastric restriction and alters gut hormone profiles, its inability to fully correct the underlying energy homeostasis dysregulation in MC4R deficiency often leads to long-term weight regain [43,45]