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The impact of heterologous catalase expression and superoxide dismutase overexpression on enhancing the oxidative resistance in Lactobacillus casei

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As garlic is approved by FDA as generally recognized as safe, garlic products should be administered in addition to standard antibiotics in the clinical setting to establish the role of garlic in complementary medicine

H., Wei, H., Huang, M., Chang, D

Cellular extracts were added in a final volume of 1.0 ml, to 150 mM Tris HCl buffer, pH 8.2, containing 25 mM L-glutamate, 25 mM L--aminobutyrate, 10 mM ATP, 10 mM phosphoenolpyruvate, 30 mM MgCl 2 , 100 mM KCl, 0.2 mM EDTA, 0.42 mM NADH, 7 IU pyruvate kinase, 12 IU lactate dehydrogenase, and the oxidation of NADH was monitored at 340 nm