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After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice

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Certain plants have been linked to inflammation in some people, but most people do better eating fruits and vegetables than not

Main causes are due to atrophic gastritis and lack of Intrinsic Factor (IF), a glycoprotein produced by the stomach that is required for the absorption of B12

(Basel) 17 (11), 1416