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Hydroxocobalamin is a powerful, long-acting form of vitamin B12 that plays a critical role in energy production, red blood cell formation, and nervous system function

Spectral measurements were made in quartz spectrophotometer cells with an optical cell path length of 1.0 mm (Starna Cells Inc.) using standard collection parameters: 20 C, 190260 nm, 0.5 nm step resolution, 50 nm/min scanning speed, 0.5 s response, 1 nm bandwidth, 8 accumulations

Statistical analysis All experimental data were expressed as mean standard deviation (x s), and comparisons between two groups were made using one-way ANOVA