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In detail, 10 L of methoxyamine (15 g/L in pyridine) was added to the dry sample that was shaken vigorously for 10 min before left to react in room temperature

The fasting duration and the time intervals between metformin gavage and subsequent procedures on the IPGTT day, as well as on the FDG injection measurement day, were the same as in the previous metformin experiment

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