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Most GI AEs were mild to moderate in severity, and there were no dose de-escalations within the first 4 weeks when transitioning from injectable therapy directly to 12 mg of orforglipron (Extended Data Fig

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14 Resulting cell suspensions were centrifuged (5 min, 300 g ) and the pellets were resuspended in DMEM (supplemented with 10% fetal calf serum and 1% antibiotics, 100 U ml 1 penicillin and 0.1 mg ml 1 streptomycin)