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In order to eliminate the precipitated protein, the supernatant was deproteinized using a 10% trichloroacetic acid (Sigma-Aldrich) solution and centrifuged at 10,000 g for 10 min at 4 C

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Antibodies against GAPDH (2118S), p-Erk1/2 (4370T), and SLC7A11 (32683S) were bought from Cell Signalling Technology (Danvers, MA)

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Available online: [PubMed] [Google Scholar] 41.Kopets R., Kuibida I., Chernyavska I., Cherepanyn V., Mazo R., Fedevych V., Gerasymov S