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The state of hepatic GH resistance in anorexia nervosa is further corroborated by the lack of increase in IGF-I following administration of supraphysiological doses of exogenous GH in women with anorexia nervosa (61), and only refeeding appears to able to restore IGF-I levels (56, 59, 62)
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At the indicated time (1, 2, 4, 6 and 22 h) an aliquot of 10 L was removed from each experimental reaction and pipetting into 100 L of 1% TFA solution to quench the reaction ( t = 0 min was determined using the reaction without enzyme)
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