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Description

Briefly, cells were washed with cold PBS, incubated with Annexin Vfluorescein isothiocyanate for 15 min and propidium iodide for 10 min at room temperature in the dark, and then analyzed by flow cytometry

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Upon ligand binding, transmembrane channels open, allowing cations such as calcium (Ca 2+ ) and sodium (Na + ) to enter the cell (Scatton 1993)

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