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Harvey CJ, Thimmulappa RK, Sethi S, et al

Following centrifugation (20,000 x g, 5 min top remove undigested material), 250 l of each sample was transferred into a new tube containing 250 l of 1 st wash buffer (50 mM TEAB, 150 mM NaCl, 0.2% NP-40) containing 50 l of streptavidin agarose beads that were pre-washed twice by the 1 st wash buffer)

Once tumours reached a mean volume of about 200 mm 3 , mice with similarly sized tumours were distributed into treatment cohorts