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The final concentrations in the well from this mixture were: 133 mM KPO 4 pH 7.5, 150 mM NaCl, 3.3 mM MgCl 2 , 4.4 mM adenosine triphosphate, 1 mM Coenzyme A (CoA), 0.1 U/mL Acetyl-CoA synthetase (ACS), 6 U/mL horseradish peroxidase, 5 U/mL Acetyl-CoA oxidase (ACO), 0.2 mg/mL fatty-acid free bovine serum albumin (BSA), 0.05 mM Amplex UltraRed, and 200 g /mL triglycerides in human VLDL