The medication is administered through injections, which are simple and can be quickly learned for self-administration
Kidneys were then excised and enzymatically digested in a solution containing 213 g/ml Liberase (Roche Diagnostics, Mannheim, Germany), 100 g/ml DNase I (Roche), and 40 g/ml collagenase IV (Gibco, Gaithersburg, MD) at 37 C for 20 min
There is no evidence of a specific withdrawal syndrome, though patients may notice increased hunger and changes in satiety signals as the medication's appetite-suppressing effects dissipate
The samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose (NC) membranes (GE Healthcare)
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