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According to NICE Clinical Knowledge Summaries, once B12 deficiency is adequately treated and symptoms resolve, routine monitoring of serum B12 levels is generally unnecessary, as clinical response is the primary indicator of treatment effectiveness

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[DOI] [PubMed] [Google Scholar] 140.Yamaguchi T, Omatsu N, Omukae A, Osumi T

The process involves: Dissolution in the stomach Binding to intrinsic factor Absorption in the ileum (small intestine) Transport through bloodstream to tissues With proper oral B12 dosage, supplementation can gradually restore normal levels, especially in cases of dietary deficiency

Subcutaneous injection is the most common research-context format today, but no published human pharmacokinetic study has formally characterized subcutaneous AOD-9604 (this gap was specifically called out in FDA's December 2024 review)