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Figure 4F illustrates that both targeting peptides significantly inhibited caspase-3 activity since decreasing fluorescence intensities of caspase-3 substrates were observed
On the 15th day after MCT post-injection, rats in ZXGD treatment groups and Sildenafil group were orally administered with different dosage of ZXGD and 35 mg/kg Sildenafil daily for consecutive 14 days
Manufactured Sterile water for laboratory peptide reconstitution
Western blot analysis Cells or purified microvesicles were lysed on ice in RIPA buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 0.1% SDS, 5 mM EDTA, 2 mM sodium pyrophosphate, 25 mM -glycerophosphate, 1% Triton X-100, 10 mM NaF, 0.5 mM DTT, 1 mM PMSF, Protease Inhibitor Cocktail), Supernatants containing the solubilized proteins were quantified by BCA Protein Assay Kit
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