In rats, GCLC has a Km for glutamate that is about 10-fold higher than that of the GCL holoenzyme, which is higher than the cellular glutamate concentration in most tissues GCL is specific for the glutamyl moiety and is regulated physiologically by: (a) non-allosteric feedback competitive inhibition by glutathione (GSH) (Ki = 2.3 mM) which involves binding of GSH to the glutamate and another site on the enzyme [8, 9] and (b) availability of its precursor, cysteine [1]
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In the realm of dysautonomia and POTS, while large-scale randomized trials isolating magnesium are scarce, there is a strong, unified clinical consensus among autonomic specialists
Previous work has reported that HGF/c-Met activation can confer hair cell protection from aminoglycoside damage, consistent with our results (Monahan and Samulski, Attenuation of aminoglycoside-induced hair cell death can result from two mechanistic categories: blockage of aminogly-coside uptake by hair cells or modulation of intracellular signaling pathways
Western blot analysis and immunoprecipitation Total cells lysates for immunoblotting or immunoprecipitation were lysed with buffer (0.1%Triton-100, 50 mM Tris-Cl, pH 7.5, 0.1% NP-40, 150 mM NaCl, 0.1 M EDTA) supplemented with a cocktail of phosphate and proteinase inhibitors for 30 min at 4 C
