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Prepare the working solutions of various components of the kit according to the method described in the previous instructions

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Methylfolate is required in the activation of tetrahydrobiopterin (i.e., BH4), which is a necessary cofactor for both tryptophan hydroxylase and tyrosine hydroxylase, enzymes required in the synthesis of serotonin and norepinephrine/dopamine, respectively (Figure 1)

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[DOI] [PubMed] [Google Scholar] 203.Park JW