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Protein expression BL21(DE3) competent cells from NEB were transformed with the respective plasmid by heat shock following the provided standard procedure of the vendor

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Refrigerate immediately after mixing Once mixed with bacteriostatic water, store the vial at 3646F (28C) right away, and use it within 28 days, the window its preservative supports

The Revised Clinical Interview Schedule (CIS-R) 82 consists of 6 topics used for interviews and was adapted as 6 nominal items to gauge overall fatigue and tiredness (range 0 to 6)

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