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Pathak treated an atherosclerotic mouse model with the mechanism-based choline TMA lyase inhibitor IMC, a drug similar to DMB, which can prevent the diet-driven accumulation of cholesterol and change the expression of hepatic genes essential for bile acid feedback regulation (49)

Rinse it with cool water

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The samples were alkylated for 45 min at room temperature, mixed with 15 L non-reducing Laemmli buffer (4% SDS, 120 mM Tris/HCl p H 6.8, 20% glycerol, 0.02% bromophenol blue) and subjected to 12% SDS PAGE