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Differentiated urothelium culture on transwell USCs were washed in PBS with 0.5 mM EDTA, trypsinized in 0.05% Trypsin and 0.5 mM EDTA for 1 min at 37 C, dissociated by vigorous pipetting, filtered through a 40 m cell strainer and resuspended in washing media

Researchers became interested in the combination after observing that glutathione levels tend to decline with age while oxidative stress, inflammation, and mitochondrial dysfunction increase.59 The idea is that providing both cysteine and glycine may help the body rebuild glutathione more efficiently than either nutrient alone