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The proteins on the PVDF membrane were blocked with 5% skim milk (1 h at room temperature), followed by an overnight incubation (4 C) with the primary antibody

After mixing the sample or standard with ThioStar and incubating at room temperature for 15 minutes, the GSH-generated signal is read at 510 nm in a fluorescent plate reader with excitation at 390 nm

The needle for a subcutaneous injection is usually small and short, so it should cause minimal discomfort

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Lion mane mushroom Hericium erinaceus contains hericenones and erinacines, bioactive compounds that stimulate NGF synthesis and appear to support BDNF expression