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To identify the sensor Cys residues of KEAP1 involved in direct cysteinylation by free Cys, we incubated the recombinant purified KEAP1 with free Cys and determined by mass spectrometry that Cys residues 77, 151, 226, 288, 319, 489, 583, and 613 were modified by cysteinylation in vitro (Fig

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