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doi:10.1085/jgp.201210772, PMID: 22585969 Sheeran FL, Rydstrm J, Shakhparonov MI, Pestov NB, Pepe S (2010) Diminished NADPH transhydrogenase activity and mitochondrial redox regulation in human failing myocardium

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The animals were then perfused with cold saline to free them of residual blood, after which liver and other tissues were collected immediately, weighed, quickly diced, and frozen in liquid nitrogen at 80 C