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2) Resuspend the cells in 1/10 volume of Lysis Buffer with appropriate amount of protease inhibitors added, which must have no effect on the binding capacity of agarose

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389 Natural compounds such as resveratrol, which induces autophagy through SIRT1 activation, 390 and spermidine, a polyamine that enhances autophagic flux, further demonstrate the therapeutic potential of autophagy induction in NDDs