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To further demonstrate activation of the TSS pathway by DMF, we investigated the metabolism of serine for de novo cysteine biosynthesis by 13 C 3 -serine tracing (Fig

The thermal program included the following melting curve steps: 10 min at 95C for 1 cycle, followed by 40 cycles for 10 sec at 95C, 20 sec at 60C and 15 sec at 72C, and then a gradual increase from 72C to 95C at 0.5C per sec

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