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doi: 10.3390/cells11121916

If you live near a Weight Watchers clinic, you can also visit in person for check-ins and weigh-ins and attend live workshops and seminars (for an extra fee)

After that, cells were washed with ice-cold PBS and lysed by 50 L lysis buffer (PBS supplemented with 20 mM Tris-HCl and 1% (v/v) Triton X-100, pH 7.4)