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The solution was then stirred for an additional 24 h at room temperature to ensure thorough conjugation

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BPC-157 acts via VEGFR2-ERK1/2 and nitric oxide pathways to promote angiogenesis and fibroblast activity, while TB-500 (the 43-amino-acid synthetic analogue of Thymosin Beta-4) functions primarily as an actin-sequestering protein that regulates G-actin/F-actin dynamics, cell migration, and endothelial differentiation