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The "energy footprint" of urban areas has always extended over increasingly large basins, shaping distant and detached territories and creating "reciprocal landscapes." For example, for much of the 20th century, hydropower production has particularly impacted alpine territories near urban areas, marginal and fragile regions both in socio-economic terms and in terms of representation and self-determination

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Tissues were washed with 0.1M sodium cacodylate and then post-fixed for 1 h at 4C in 1% osmium tetroxide (Electron Microscopy Sciences) in 0.1M sodium cacodylate