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GHK in human plasma declines from approximately 200 ng/mL at age 20 to under 80 ng/mL by age 60

Pickart originally isolated GHK as the "growth factor" responsible for restoring proliferation to liver cells in older-donor plasma, and the copper-binding role was recognized shortly afterward as the molecular basis for many of the activities attributed to the peptide

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Despite this complexity, biogenic ice nucleation has been modeled as a single INP type with homogeneous properties 40