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Iron dosage For immunofluorescence staining, cells were plated and grown in the appropriate medium supplemented with 2% FBS 24 h before being treated as indicated

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Tissue homogenates were transferred into calibrated Oxygraph-2k (O2k, Oroboros Instruments), and DatLab software (v.7.4, Oroboros Instruments) was used to record real-time oxygen concentration (M), as well as oxygen flux per tissue mass (pmol O 2 s 1 mg 1 )