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Flow cytometry quantification of cell viability of HC lymphocytes, monocytes, and neutrophils cultured with 20% HC or SLE serum for 16 hours and the proportion of apoptotic (Annexin V + 7AAD-), necrotic (Annexin V + 7AAD + ) and live (Annexin V- 7AAD-) cells in each subset was analyzed (n = 6)

[DOI] [PMC free article] [PubMed] [Google Scholar] 50.Chang YH, Chang DM, Lin KC, Shin SJ, Lee YJ

M.Aranda-DazA.TropiniC.FrankelM

Enhanced Production of a Thermostable Carbonic Anhydrase in Escherichia Coli by Using a Modified NEXT Tag

qRT-PCR analyses were performed using 2x qPCRBIO Sybr Green blue Mix (PCR Biosystem) on a Real-Time PCR Detection System (Applied Biosystems)