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2.12 Measurement of GLP-1R compartmentalization by BRET HEK293T cells in 6-well plates were transfected with 0.2 g SNAP-GLP-1R-NLuc, 0.2 g of KRAS-Venus, Rab5-Venus, Rab7-Venus or Rab11-Venus (kindly provided by Prof Nevin Lambert, Medical College of Georgia, and Prof Kevin Pfleger, University of Western Australia), plus 1.6 g pcDNA3.1, 24 hours before the assay