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The beads were then washed with His-wash buffer A (50 mM NaHPO, 50 mM NaHPO (pH 8.0), 400 mM NaCl, 60 mM imidazole, and 0.1% Tween 20)

The loss of the chondrocytic phenotype and proliferative potential during in vitro expansion, termed as dedifferentiation, has been well documented

Plant and Soil 150 : 41-49

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Glutathione peroxidase 4 (GPX4) plays a central role in counteracting ferroptosis by reducing membrane lipid hydroperoxides to their corresponding alcohols, utilizing glutathione (GSH) as a reducing substrate (15)