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DPP-4 enzyme assays The enzyme assay was performed in flat-bottom 96- well microplates, where each reaction consisted of 100 L of either 12.5% human, platypus or echidna serum, 0.5 mM of H-Ala-Pro-p-nitroanilide (Bachem) as a substrate in the presence/absence of 100 M of DPP-4 inhibitor P32/98 (Santa Cruz Biotech) in 100 mM sodium phosphate buffer (PH 7.4) supplemented with 150 mM NaCl and 0.2% Tween-20 with a total volume of 200 M

Knockdown of STAT3 or use of the STAT3 inhibitor W1131 can induce ferroptosis through Fe 2+ accumulation and lipid peroxidation in gastric cancer cells

Diabetologia 46 , 250254 (2003)

(1) This guide will explore the science of protein and its immune-strengthening benefits, helping you make an informed choice about the best protein powder for your individual health goals

Lupus 11 , 865871 (2002)