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To isolate single-cell clones, antibiotic-selected polyclonal GGT1 KO cells were seeded at low density (50 cells per 15 cm dish) and grown for 2128 days

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Exercise training can enhance BCAA oxidative capacity, reduce circulating BCAA levels, and improve insulin sensitivity without causing nutritional deficiency ( 5.4 Genetic and epigenetic factors 5.4.1 BCKDH gene polymorphisms The BCKDH complex is the rate-limiting enzyme of BCAA catabolism, and genetic variations in this complex significantly affect individual BCAA metabolic capacity