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Patients were randomized to TRESIBA U-200 once-daily with the evening meal or insulin glargine U-100 once-daily according to the approved labeling

Determination of cell death by PI staining Cells were trypsinized, washed with PBS and suspended in 300 l Dulbeccos phosphate buffered saline (DPBS) supplemented with 0.2% BSA containing 0.3 g/ml propidium iodine (PI, Sigma-Aldrich)

Omega-3: Omega-3s are important to cells, provide the body with energy, and support the health of many body systems

Consideration of individual patient factors, overall health, liver function, and specific medication formulations/routes is crucial