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doi: 10.3390/genes10030236 149 SakkasDAlvarezJG

For this purpose, 4 l of reagent B was preliminarily applied to the tube wall above the level of reagent A, and then the biomaterial (according to the SUM and NEO schemes) or trichloroacetic acid (according to schemes X and ST) was added, carefully pipetting, mixing, and rinsing the walls with a mixture of reagents

From a clinical perspective, large-scale, longitudinal studies are needed to evaluate the robustness of GBB and L-CN as early biomarkers across diverse populations, considering inter-individual variability in microbiota composition, diet, and genetic background