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A standard competitive binding assay protocol using 125 I-NDP-MSH as the radioligand was followed

Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems

RA provides a critical signal for iTreg cell differentiation and iTreg cells can inhibit Th1- and Th17-type inflammatory responses (1921)

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