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In the reaction mixtures without enzyme, varying amounts of MBB (0-300 l of 1 mM stock solution which gave final concentration 0-100 M) were added into different fluorescent cuvettes already containing 3.0 ml of phosphate buffer (pH 6.5) and 100 l of GSH stock solution (3 mM)

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In this group, 9% used the medications specifically to treat a chronic condition such as heart disease or diabetes, the highest rate of medical use across all age groups