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Functional activation of DCs stimulates NK-cells, promotes an increase in proinflammatory cytokines (IL-12, IL-6, IL-23, IL-1) and a decrease in antiinflammatory transforming growth factor- (TGF-1), which control adaptive reactions, which contributes development of Th1 and Th17, IL-17-producing T cells and cytotoxic T cells

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Proteins were transferred to PVDF membranes at 400 mA constant current for 90 min in ice-cold transfer buffer