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After blocking with 5% nonfat milk, membranes were incubated with primary antibodies overnight at 4 C

If you're using a different concentration (e.g., compounded tirzepatide at 5 mg/mL or 20 mg/mL), the unit count changes proportionally

Acknowledgements The authors would like to thank all the participants, investigators and trial site staff who were involved in the conduct of the trials

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