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The change in molar ellipticity observed in the range from ~210 to ~250 nm, with a maximal change at 222 nm, clearly indicates that the MT2 conformation, which is hardly observable in CD spectra, changes during the reaction due to Zn(II) dissociation and Cys residue alkylation
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About 40% of patients with diabetes could develop CKD [4], and it could be already present at diagnosis especially in patients with type 2 diabetes [5]
While pharmacologic inhibition may not induce enough inhibition, G6PD knockdown may go too far, and neither recapitulates the protein instability that is characteristic of human G6PD-d
The residue was resuspended with 25 L of a solution consisting of 90% acetonitrile and 10% 20 mM ammonium formate at pH 3.5 (v/v)
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