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The cells were washed, resuspended, and analyzed by flow cytometry (Afla Aesar, Beckman, USA)

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Telomere-regulating genes and the telomere interactome in familial cancers

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Briefly, 20 ng of the wildtype human GIP receptor (E354 variant) was added to 2x KAPA HiFi HotStart ReadyMix, 0.3 M of forward and reverse primers and DNAse-free water to make a final reaction volume of 25 L