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Protein concentration was determined using a BCA kit (Thermo Scientific), after which samples were diluted to 1 mg ml 1 in 5% SDS and 50 mM TEAB

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It acts as a mediator that converts these harmful elements into water-soluble forms and thus enables them to be excreted via the kidneys

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The second class of Grxs, known as class II or monothiol Grxs, features a consensus CGFS active site motif