Moran LJ, Ko H, Misso M, Marsh K, Noakes M, Talbot M, Frearson M, Thondan M, Stepto N, Teede HJ
Intra-NTS Ex-4 in rats increased dopamine-B-hydroxylase expression in NTS, an enzyme for noradrenaline synthesis, and GLP-1 fibers and noradrenergic neurons were found to be colocalized in the NTS (Richard et al., 2015)
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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step
doi: 10.1001/jamadermatol.2018.3359 62 ClarkWHJr.ReimerRRGreeneMAinsworthAMMastrangeloMJ