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Cell pellets were collected by centrifugation at 300 g for 5 min, then were fixed by Carnoys solution (methanol: glacial acetic acid =3:1, v/v) for 20 min at RT and at 4 C for overnight

Particular emphasis is placed on the key technologies enabling the transition from chemical synthesis to scalable biomanufacturing, including tandem-repeat expression, high-cell-density fermentation, inclusion-body-assisted purification, and Cu(II) complex stabilization

Antioxidant effect Moreover, the homocysteine transsulfuration through a metabolic process by methionine results in cysteine, which becomes glutathione

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